Composite
Part:BBa_K241000:Design
Designed by: John Heil Group: iGEM09_Waterloo (2009-10-20)
Nuclease construct, no sense promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1497
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1599
Illegal BsaI.rc site found at 223
Illegal BsaI.rc site found at 912
Illegal BsaI.rc site found at 1292
Design Notes
The main design consideration was to have maximal repression of the toxic nuclease genes.
Source
Synthetic, Phage T7, Phage Lambda, Pseudomonas mendocina.