Composite

Part:BBa_K241000:Design

Designed by: John Heil   Group: iGEM09_Waterloo   (2009-10-20)


Nuclease construct, no sense promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1599
    Illegal BsaI.rc site found at 223
    Illegal BsaI.rc site found at 912
    Illegal BsaI.rc site found at 1292


Design Notes

The main design consideration was to have maximal repression of the toxic nuclease genes.


Source

Synthetic, Phage T7, Phage Lambda, Pseudomonas mendocina.

References